Creation of a Bioengineered Ovary: Isolation of Female Germline Stem Cells for the Repopulation of a Decellularized Ovarian Bioscaffold

Ovarian failure is the most common cause of infertility and affects about 1% young women. One innovative strategy to restore ovarian function may be represented by development a bioprosthetic ovary, obtained through combination tissue engineering regenerative medicine.We here describe two main steps required for bioengineering ovary its ex vivo functional reassembling. The first step aims at producing 3D bioscaffold, which mimics natural milieu in vitro. This with whole organ decellularization technique that allows maintenance microarchitecture biological signals original tissue. second involves use magnetic activated cell sorting (MACS) isolate purified female germline stem cells (FGSCs). These are able differentiate adult mature cells, when subjected specific stimuli, can used them repopulate decellularized bioscaffolds. techniques represents powerful tool vitro recreation bioengineered constitute promising solution hormone fertility restoring. In addition, procedures described allow creation suitable platform useful applications both toxicological transplantation studies.